Chromatography Media
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Filtered Search Results
Cytiva TALON™ Superflow Medium
Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | Talon Superflow Media |
| Binding Capacity | Up to 20 mg Histidine-tagged Protein |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography |
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva Capto™ Multimodal (MMC) Chromatography Media
Used to solve specific purification challenges at both high and low ionic strengths
| Particle Size | 75 μm |
|---|---|
| Product Type | Capto MMC Media |
Cytiva HiTrap™ AVB Sepharose™ Columns
Enables purification of adeno associated viruses (AAV) in one step, with high purity and excellent yields. Cytiva HiTrap™ AVB Sepharose™ Columns are pre-packed with affinity chromatography medium (resin) for simple operation with a syringe, pump or chromatographic system.
| Particle Size | 24 to 44 μm |
|---|---|
| Ligand Type | 14 kD recombinant protein |
| Flow Rate | <150 cm/hr |
| Matrix | Cross-linked agarose |
| Stability | Stable to all commonly used aqueous buffers |
| pH Range | 3 to 10 |
| Product Type | HiTrap AVB Sepharose HP Column |
| Binding Capacity | >10▿12 genecopies/mL Chromatography Mediμm |
| Material | Polypropylene |
| Storage Requirements | Store prepacked column at 4°C to 30°C in 20% Ethanol and Media at 4°C to 8°C in 20% Ethanol. |
| For Use With (Application) | One Step Purification of Adeno Associated Viruses |
| Height (Metric) Bed | 25 mm |
| Max. Pressure | 70 psi (5 bar) |
Cytiva DEAE Sepharose™ Fast Flow Chromatography Media
For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.
Cytiva PlasmidSelect Xtra Medium
PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography resin with a selectivity that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
| Particle Size | 24 to 44 μm |
|---|---|
| Ligand Type | 2-mercaptopyridine |
| Matrix | Cross-linked Agarose |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 3 to 11 |
| Product Type | PlasmidSelect Xtra Media |
| Grade | BioProcess resin |
| Storage Requirements | 4 to 30 deg.C, 20% Ethanol |
| For Use With (Application) | Generic Purification of Supercoiled Plasmid DNA |
| Max. Pressure | <120 cm/h |
Cytiva Phenyl Sepharose™ High Performance Media
Designed for intermediate and polishing step purification steps
Cytiva Octyl Sepharose™ 4 Fast Flow Chromatography Media
Aliphatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification of larger proteins
| Particle Size | 45 to 165 μm |
|---|---|
| Ligand Type | Octyl |
| Matrix | 4% Cross-Linked Agarose |
| Stability | Stable in commonly used aqueous Buffers: 1 M NaOH, 30% Isopropanol, 70% Ethanol, 6 M Guanidine-Hydrochloride, 30% Acetonitrile, 1 mM HCl |
| Storage Buffer | 20% Ethanol |
| pH Range | 2 to 14 |
| Product Type | Octyl Sepharose 4 Fast Flow Media |
| Storage Requirements | 4°C to 30°C |
| Max. Pressure | 100 kPa |
Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media
Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.
| Particle Size | 90 μm |
|---|---|
| Ligand Type | Native Protein A (S. aureus) |
| Flow Rate | 150 to 250 cm/Hoμr |
| Matrix | 4% Cross-Linked Agarose |
| Stability | No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol |
| Storage Buffer | 20% Ethanol |
| Product Type | nProtein A Sepharose 4 Fast Flow Media |
| Binding Capacity | >35 mg Human IgG/mL |
| Height (English) Bed | 9.84 in. |
| Storage Requirements | 2°C to 8°C |
| Height (Metric) Bed | 25 cm |
| Max. Pressure | 100 kPa |
Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media
USe for separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Cytiva Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes.
Cytiva Butyl Sepharose™ High Performance Chromatography Media
Designed for intermediate and polishing step purification steps
| Particle Size | 24 to 44 μm |
|---|---|
| Ligand Type | Butyl |
| Flow Rate | 100 to 200 cm/Hoμr |
| Matrix | Cross-Linked Agarose |
| Stability | Stable in commonly used aqueous Buffers: 1 M Sodium Hydroxide, 1 M Acetic Acid, 8 M Urea, 6 M Guanidine Hydrochloride, 30% Acetonitrile, 70% Ethanol, 3 M Ammonium sulfate, 1 mM HCl, 30% Isopropanol, 2% SDS |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 13 |
| Product Type | Butyl Sepharose High Performance Media |
| Binding Capacity | 38 mg b-lactoglobulin/mL mediμm |
| Storage Requirements | 4°C to 30°C |
| For Use With (Application) | For Intermediate and Polishing Step PurifiCatIon Steps |
| Max. Pressure | 300 kPa |
Cytiva SP Sepharose™ High Performance Media
Use for high intermediate and polishing steps in downstream processing. Cytiva Life Sciences™ SP Sepharose™ High Performance Media is composed of crosslinked agarose beads.
Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media
Used for purifying proteins and peptides that have an affinity for metal ions. Cytiva IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
| Product Type | IMAC Sepharose 6 Fast Flow Media |
|---|---|
| For Use With (Application) | For Purifying Proteins and Peptides That Have an Affinity for Metal Ions |
Cytiva SOURCE™ 15S Chromatography Media
Chemically stable cation exchanger for high resolution polishing purification of proteins, peptides and oligonucleotides
| Stability | Stable in common Ion Exchange Buffers |
|---|---|
| Storage Buffer | 20% Ethanol, 0.2 M Sodium Acetate |
| pH Range | 1 to 14 |
| Height (English) Bed | 3.93 in. |
| Storage Requirements | 4°C to 30°C |
| For Use With (Application) | For High Resolution Polishing PurifiCatIon of Proteins, Peptides and Oligonucleotides |
| Max. Pressure | 100 kPa |
| Particle Size | 15 μm |
| Ligand Type | Sulphonate Group |
| Flow Rate | 400 cm/Hoμr |
| Matrix | Polystyrene/Divinylbenzene |
| Product Type | Source 15 S Media |
| Ion Exchange Type | Strong CatIon Exchanger |
| Height (Metric) Bed | 10 cm |
Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media
Used for capture and intermediate purification requiring low to medium hydrophobicity
| Ligand Type | Phenyl |
|---|---|
| Matrix | 6% Cross-Linked Agarose |
| pH Range | 3 to 13 |
| For Use With (Application) | For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity |